- 商品介绍
- 规格参数
- 包装参数
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规格 |
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| Bacterial or Yeast Strain: | DH5α |
| Blue/White Screening: | Yes |
| Cloning Methylated DNA: | No |
| Cloning Unstable DNA: | Not Suitable for cloning unstable DNA |
| Competent Cell Type: | Chemically Competent |
| F'episome (to make ssDNA): | Lacks F' Episome |
| High-throughput Compatibility: | Not High-throughput Compatible (Manual) |
| Preparing Unmethylated DNA: | Not Suitable for preparing unmethylated DNA |
| Product Line: | DH5a, Library Efficiency® |
| Propagating ccdB Vectors: | Not for ccdB vector propagation |
| Reduces Recombination: | Yes |
| Shipping Condition: | Dry Ice |
| T1 Phage - Resistant (tonA): | No |
| Transformation Efficiency: | > 1x10^8 |
| Transformation Efficiency Level: | Medium Efficiency (10^8-10^9 cfu⁄µg) |
| Format: | Tube(s) |
| Improves Plasmid Quality: | Yes |
| Species: | E. coli |
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储存 |
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Contains: • Library Efficiency DH5α Competent Cells: 5 vials, 200 µl each (total of 1 ml) • pUC19 DNA (10 pg/ul): 1 vial, 50 µl • S.O.C. medium: 2 bottles, 6 ml each Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4C or at Room Temperature. |
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描述 |
Library Efficiency DH5α Competent Cells are a versatile strain of chemically competent cells that demonstrate transformation efficiencies of >1 x 108 cfu/µg plasmid DNA. Library Efficiency DH5α Competent Cells are ideal for difficult cloning constructions or any application that uses limiting amounts of DNA. Features of these cells:
• Supports blue/white screening by α-complementation on plates containing X-Gal or Bluo-Gal
• Maintains plasmid DNA with minimum recombination events
• Delivers high-yield plasmid preparations for downstream applications
• M13mp cloning vectors may be propagated on a lawn of DH5α-FT, DH5αF', DH5αF'IQ, JM101, or JM107 for plaque formation
Ideal for difficult cloning projects
Library Efficiency DH5α Competent Cells are recommended for routine cloning of genes into large plasmids, and are suitable for difficult clone constructions, such as blunt-end ligations. Library Efficiency DH5α Competent Cells have a mid-range transformation efficiency (>1 x 108 cfu/µg control DNA per 100 µL reaction). The strain also has the Φ80lacZΔM15 genotype, allowing blue/white screening on plates containing either X-Gal or Bluo-Gal. Finally, the recA1 mutation helps reduce the rate of recombination while propagating plasmid DNA, and the endA1 mutation makes DH5α cells an excellent choice for amplifying plasmid DNA for subsequent extraction and purification.
Genotype: F- Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17(rk-, mk+) phoA supE44 thi-1 gyrA96 relA1 λ-
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