规格
|
Glucose:
|
High Glucose |
HEPES Buffer:
|
No HEPES |
Product Line:
|
BenchStable™ |
Serum Supplementation:
|
Standard Serum Supplementation |
Shipping Condition:
|
Room Temperature |
Sodium Pyruvate Additive:
|
Sodium Pyruvate |
Form:
|
Liquid |
Glutamine:
|
GlutaMAX™-I |
Phenol Red Indicator:
|
Phenol Red |
Quantity:
|
10 x 500 mL |
储存
|
Store at room temperature. Protect from light. |
Gibco DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
BenchStable DMEM/F-12 has been engineered for storage at room temperature while supporting cellular proliferation and maximum cell densities comparable to conventional DMEM/F12 when supplemented with ≥10% fetal bovine serum (FBS).
This DMEM/F-12 is modified as follows:
• Optimized for room temperature stability
• With GlutaMAX, Phenol Red
• Without HEPEPS
Using BenchStable DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH. DMEM/F-12 with GlutaMAX supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. BenchStable DMEM/F-12 contains no proteins, lipids, or growth factors and so requires supplementation with ≥10% fetal bovine serum (FBS).
cGMP manufacturing and quality system
Gibco BenchStable media is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
For more information on Gibco BenchStable media, please visit thermofisher.com/benchstable.