Alexa Fluor® 750 Carboxylic Acid is a non-reactive form of Alexa Fluor® 750 that can be used as a reference standard for dye-conjugates. Additionally, this carboxylic acid derivative can be converted to an amine-reactive ester using standard chemical techniques or coupled to hydrazines, hydroxylamines, and amines in aqueous solution using water-soluble carbodiimides such as EDAC (Cat. No. E2247).

Characteristics of Alexa Fluor® 750 dye include:

• Laser line: 750 nm
• Common filter set: Cy® 7
• Excitation maxima: 749 nm
• Emission maxima: 775 nm

More About Alexa Fluor® 750 Dye
Alexa Fluor® 750 dye and its conjugates exhibit fluorescence spectra that are very similar to those of CY® 7 dye. Its fluorescence emission maximum at 775 nm is well separated from commonly used far-red fluorophores such as Alexa Fluor® 647 or allophycocyanin (APC), facilitating multicolor analysis.

Discover the Benefits of the Alexa Fluor® Dye Series
Compared to traditional dyes, Alexa Fluor® dyes are brighter, more photostable, and more pH-resistant between pH 4 and 10. And generally when using Alexa Fluor® dyes, higher degrees of labeling can be achieved without intramolecular quenching. For details see Alexa Fluor® Dyes Spanning the Visible and Infrared Spectrum in the Molecular Probes Handbook.

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