StainExpress™ Immune Cell composition Cocktail helps standardize the flow cytometric evaluation of immune cell fractions and composition from human blood material. This pre-formulated multicolor panel is designed for immunofluorescent staining of whole blood, peripheral mononuclear cells (PBMCs), lysed whole blood samples, or other single-cell suspensions from human tissue, to identify human monocytes, neutrophils, eosinophils, and T, B, and NK lymphocyte populations as well as CD4⁺, CD8⁺, and NKT cell subsets.

Background information

The StainExpress Immune Cell Composition Cocktail is designed for standardized determination of cell viability and composition of immune cells, helping to achieve reliable results for raw material testing, in-process, and quality control steps in cell manufacturing as well as reproducible analysis on human whole blood. The cocktail offers consistent formulation in carefully dried down cocktails containing our REAfinity™ Recombinant Antibodies.

REAfinity Antibodies are generated in a biologically and chemically defined in vitro expression system to guarantee maximum purity and lot-to-lot consistency. The genetic sequence is engineered to lack binding to Fcγ receptors (FcγRs). Binding to FcγRs is one of the main causes of background signal in flow cytometry analysis.

Flow cytometric analysis of the Immune Cell Composition Cocktail can be conducted on several flow cytometer equipped with a red (638 nm), a blue (488 nm), and a violet (405 nm) laser, for example, the MACSQuant^® Analyzer 10 or MACSQuant Analyzer 16.

Applications

Evaluation of leukocyte subsets in whole blood, leukapheresis products, PBMCs, or other single-cell suspensions from human tissue
Determination of cell counts and population subsets during CAR T cell manufacturing procedures using the MACSQuant Analyzer

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