ALEXA FLUOR 546 FREE ACID 5 mg，A33079，Invitrogen

Alexa Fluor® 546 Carboxylic Acid is a non-reactive form of Alexa Fluor® 546 that can be used as a reference standard for dye-conjugates. Additionally, this carboxylic acid derivative can be converted to an amine-reactive ester using standard chemical techniques or coupled to hydrazines, hydroxylamines, and amines in aqueous solution using water-soluble carbodiimides such as EDAC (Cat. No. E2247).

Characteristics of Alexa Fluor® 546 dye include:

• Laser line: 546 nm
• Common filter set: TRITC (tetramethylrhodamine)
• Excitation maxima: 556 nm
• Emission maxima: 573 nm

More About Alexa Fluor® 546 Dye
Alexa Fluor® 546 dye is a bright, yellow-fluorescent dye that can be excited using the 488 nm or 532 nm laser lines. Alexa Fuor® 546 serves as an alternative for tetramethylrhodamine or Cy®3 dye, with higher initial intensity than Alexa Fluor® 555 dye, but lower photostability in buffer or antifade. Learn more about this dye and related products.

Discover the Benefits of the Alexa Fluor® Dye Series
Compared to traditional dyes, Alexa Fluor® dyes are brighter, more photostable, and more pH-resistant between pH 4 and 10. And generally when using Alexa Fluor® dyes, higher degrees of labeling can be achieved without intramolecular quenching. For details see Alexa Fluor® Dyes Spanning the Visible and Infrared Spectrum in the Molecular Probes Handbook.