MAX Efficiency™ Stbl2™ Competent Cells ，10268019，Invitrogen

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订货号： 3QR1748

品牌型号： Invitrogen 10268019

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最小订货量： 1套

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商品介绍
规格参数
包装参数

产品介绍 Product Description

规格
Bacterial or Yeast Strain:	Stbl2™
Blue/White Screening:	No
Cloning Methylated DNA:	Yes
Cloning Unstable DNA:	Suitable for cloning unstable DNA
Competent Cell Type:	Chemically Competent
F'episome (to make ssDNA):	Lacks F' Episome
High-throughput Compatibility:	Not High-throughput Compatible (Manual)
Plasmid:	May be used for plasmids > 20 kb
Preparing Unmethylated DNA:	Not Suitable for preparing unmethylated DNA
Product Line:	MAX Efficiency™, Stbl2™
Propagating ccdB Vectors:	Not for ccdB vector propagation
Reduces Recombination:	Yes
Shipping Condition:	Dry Ice
T1 Phage - Resistant (tonA):	No
Transformation Efficiency:	> 1x10^9
Transformation Efficiency Level:	High Efficiency (> 10^9 cfu⁄µg)
Format:	Tube(s)
Improves Plasmid Quality:	Yes
Species:	E. coli

储存
Contains: • MAX Efficiency® Stbl2™ Competent Cells: 5 vials, 200 µl each (total of 1 ml) • pUC19 DNA (0.01 µg/ml): 1 vial, 100 µl • SOC Medium: 1 bottle, 6 ml Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.

描述

MAX Efficiency® Stbl2™ Competent Cells are high-efficiency chemically competent cells specifically designed for cloning unstable inserts. In addition to recA1, a unique set of genetic markers allow for stable cloning of direct repeat and retroviral sequences and tandem array genes. MAX Efficiency® Stbl2™ Competent Cells offer:

• >1 x 10⁹ transformants/µg plasmid DNA for cloning rare sequences
• Elimination of mcrBC-hsdRMS-mrr and mutation of mcrA—allows cloning of methylated genomic sequences
• A unique genotype that stabilizes direct repeat and retroviral sequences such as SIV and HIV
• Greatly increased plasmid yield and quality due to endA1 mutation

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