- 商品介绍
- 规格参数
- 包装参数
规格 |
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Bacterial or Yeast Strain: | Stbl2™ |
Blue/White Screening: | No |
Cloning Methylated DNA: | Yes |
Cloning Unstable DNA: | Suitable for cloning unstable DNA |
Competent Cell Type: | Chemically Competent |
F'episome (to make ssDNA): | Lacks F' Episome |
High-throughput Compatibility: | Not High-throughput Compatible (Manual) |
Plasmid: | May be used for plasmids > 20 kb |
Preparing Unmethylated DNA: | Not Suitable for preparing unmethylated DNA |
Product Line: | MAX Efficiency™, Stbl2™ |
Propagating ccdB Vectors: | Not for ccdB vector propagation |
Reduces Recombination: | Yes |
Shipping Condition: | Dry Ice |
T1 Phage - Resistant (tonA): | No |
Transformation Efficiency: | > 1x10^9 |
Transformation Efficiency Level: | High Efficiency (> 10^9 cfu⁄µg) |
Format: | Tube(s) |
Improves Plasmid Quality: | Yes |
Species: | E. coli |
储存 |
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Contains: • MAX Efficiency® Stbl2™ Competent Cells: 5 vials, 200 µl each (total of 1 ml) • pUC19 DNA (0.01 µg/ml): 1 vial, 100 µl • SOC Medium: 1 bottle, 6 ml Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature. |
描述 |
MAX Efficiency® Stbl2™ Competent Cells are high-efficiency chemically competent cells specifically designed for cloning unstable inserts. In addition to recA1, a unique set of genetic markers allow for stable cloning of direct repeat and retroviral sequences and tandem array genes. MAX Efficiency® Stbl2™ Competent Cells offer:
• >1 x 109 transformants/µg plasmid DNA for cloning rare sequences
• Elimination of mcrBC-hsdRMS-mrr and mutation of mcrA—allows cloning of methylated genomic sequences
• A unique genotype that stabilizes direct repeat and retroviral sequences such as SIV and HIV
• Greatly increased plasmid yield and quality due to endA1 mutation
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