规格
Final Product Type:	cDNA (Labeled)
Includes Label or Dye:	Yes
Labeling Method:	Direct Labeling
Labeling Target:	cDNA
Label or Dye:	Alexa Fluor™ 555, Alexa Fluor™ 647
Product Line:	Alexa Fluor™, SuperScript™
Sample Type:	RNA
Detection Method:	Fluorescent

储存

All SuperScript® Direct cDNA Labeling Systems/Modules include SuperScript® III Reverse Transcriptase, anchored Oligo(dT), random primers, control RNA, 5X first-strand buffer, 0.1 M DTT, 10 mM dNTP mix, low-elution purification columns, loading buffer, wash buffer, amber collection tubes, and DEPC-treated water. The SuperScript® Plus Direct cDNA Labeling System/Module additionally includes Alexa Fluor® 555- and 647-aha-dUTP. The 'Module' does not include purification components. For purification components, see the 'System' (L101506).

Store purification columns, loading buffer, and wash buffer at room temperature. Store all other components at -20°C. Guaranteed stable for 6 months when properly stored.

描述

The SuperScript® Plus Direct cDNA Labeling System is a microarray labeling kit that combines the superior performance of SuperScript® III Reverse Transcriptase (RT) with the simplicity of direct labeling. In place of an enzyme spike-in, the streamlined protocol incorporates balanced sets of novel, array-optimized, nucleotides conjugated to Alexa Fluor® 555 and 647 dyes. This results in more reproducible array data with higher correlation coefficients than using standalone reagents. The SuperScript® Plus Direct cDNA Labeling System provides:

• Optimized, all-inclusive direct labeling with balanced aha-Alexa Fluor® nucleotides, enabling higher numbers of array positives
• Improved correlation coefficients for more accurate data (Figure 1)
• Low elution purification columns included for ease of use

In the SuperScript® Plus cDNA Labeling method, anchored oligo(dT) anneals to the mRNA template (Figure 2). SuperScript® III RT extends from the priming site, incorporating dUTP labeled with Alexa Fluor® dye directly to produce labeled cDNA. Template RNA is degraded by base hydrolysis. Free nucleotides and other contaminants are removed using low-elution volume purification columns before hybridization to the microarray.

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