规格
Bacterial or Yeast Strain:	DH10B
Blue/White Screening:	Yes
Cloning Methylated DNA:	Yes
Cloning Unstable DNA:	Not Suitable for cloning unstable DNA
Competent Cell Type:	Electrocompetent
F'episome (to make ssDNA):	Lacks F' Episome
High-throughput Compatibility:	Not High-throughput Compatible (Manual)
Plasmid:	May be used for plasmids > 20 kb
Preparing Unmethylated DNA:	Not Suitable for preparing unmethylated DNA
Product Line:	DH10B, ElectroMAX™
Propagating ccdB Vectors:	Not for ccdB vector propagation
Reduces Recombination:	Yes
Shipping Condition:	Dry Ice
T1 Phage - Resistant (tonA):	Yes
Transformation Efficiency:	> 1x10^10
Transformation Efficiency Level:	High Efficiency (> 10^9 cfu⁄µg)
Format:	Tube(s)
Improves Plasmid Quality:	Yes
Species:	E. coli

储存
Contains: • ElectroMAX DH10B T1 Phage-Resistant Cells: 5 vials, 100 µl each • pUC19 DNA (10 pg/µl): 1 vial, 50 µl • S.O.C. medium: 2 bottles, 6 ml each Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.

描述

ElectroMAX DH10B T1 Phage-Resistant Competent Cells offer transformation efficiencies that are among the highest achieved by any product we offer, and can exceed 1 x 10¹⁰ cfu/µg plasmid DNA in efficiency. They are ideal for applications requiring high-efficiency transformation, including cDNA and gDNA library construction. ElectroMAX DH10B cells:

• Maximize yield of transformants from limited cloning products
• Permit efficient cloning of methylated DNA
• Are resistant to T1 and T5 bacteriophages
• Support blue/white screening by α-complementation on plates containing X-Gal or Bluo-Gal
• Deliver high-yield plasmid preparations for downstream applications

Phage-resistant representative libraries from a single transformation
The tonA mutation in DH10B cells confers resistance to bacteriophages T1 and T5, safeguarding valuable libraries against contamination. Additionally, mutations in the methylation-dependent restriction system (mcrA, mcrBC, and mrr) make ElectroMAX DH10B T1 Phage-Resistant Cells ideal for construction of genomic libraries of both prokaryotic and eukaryotic genomic DNA, and allow for efficient plasmid rescue from eukaryotic genomes. ElectroMAX DH10B T1 Phage-Resistant Cells are suitable for construction of gene banks, for the generation of cDNA libraries using plasmid-derived vectors, and for situations when DNA is limiting. This strain also has the Φ80lacZΔM15 genotype, providing for the option of blue/white screening on plates containing either X-Gal or Bluo-Gal. Finally, the endA1 mutation makes DH10B an excellent vehicle for amplifying plasmid DNA for subsequent extraction and purification.

Genotype: F^-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139Δ(ara, leu)7697 galU galK λ^-rpsL nupG tonA

Find the strain and format that you need
DH10B cells are available in both electrocompetent and chemically competent formats. Our ElectroMAX DH10B cells are also suitable for cDNA or gDNA library construction but are not resistant to T1 and T5 phage infection. Our MegaX DH10B T1^R Electrocomp Cells have the highest transformation efficiency (>3 x 10¹⁰ cfu/µg plasmid DNA) and also offer the benefit of T1 and T5 phage resistance.

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