规格
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Cell Permeability:
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Cell-Permeant |
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Detection Method:
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Fluorescent |
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For Use With (Equipment):
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Fluorescence Microscope, Flow Cytometer, Microplate Reader |
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Indicator:
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pH Indicator |
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Indicator Properties:
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Fluorescent Dye-Based |
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Shipping Condition:
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Room Temperature |
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Concentration:
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1 mg⁄ml |
储存
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Store in freezer (-5 to -30°C). |
BCECF, AM is a cell-permeant, dual-excitation ratiometric pH indicator with a pKa of ~6.98 and is ideal for measuring changes in the cytosolic pH of most cells. Intracellular pH measurements with BCECF are made by determining the pH-dependent ratio of emission intensity (detected at 535 nm) when the dye is excited at ~490 nm versus the emission intensity when excited at its isobestic point of ~440 nm.