规格
|
Assay Entry:
|
Cell-based terbium modification activity (transient), Cell-based terbium phospho-specific activity (transient) |
Cell State:
|
Cells Not Included |
Druggable Target:
|
Kinases |
Human Protein Class:
|
Kinases, Cell Signalling |
Product Line:
|
LanthaScreen® |
Readout:
|
End Point |
Reporter Gene:
|
GFP (EmGFP) |
System Type:
|
LanthaScreen® |
Target Entry:
|
AKT1 |
Technique:
|
TR-FRET |
Detection Method:
|
Fluorescent |
储存
|
Kit contains Terbium-labeled anti-AKT antibody, BacMam AKT Virus, and 6X Lysis Buffer. Store as indicated in handling documentation. |
The combination of baculovirus-mediated gene delivery (BacMam) with LanthaScreen® Cellular Assay technology enables a platform for the analysis of specific posttranslational modifications of various substrates including AKT. BacMam provides a convenient genetic delivery tool for a GFP-AKT fusion protein in the cell line of interest. This kit describes an HTS-compatible cellular immunoassay measuring phosphorylation of GFP-AKT at Thr308.