储存
Sufficient For: 1000 cm² of membrane (10 mini-gel blots) • Stabilized Streptavidin-HRP, 1.5 mL • Luminol/Enhancer Solution, 80 mL • Stable Peroxide Solution, 80 mL • Hybridization Buffer, 125 mL • Hybridization Stringency Wash Buffer (2X), 375 mL • Blocking Buffer, 500 mL • Wash Buffer (4X), 500 mL • Substrate Equilibration Buffer, 500 mL Upon receipt, store kit at 4°C.

描述

Thermo Scientific North2South Chemiluminescent Detection Kit combines a novel ECL substrate for horseradish peroxidase (HRP) and optimized hybridization and blocking buffers to ensure consistent, high-sensitivity Southern blot results.

Features of the North2South Chemiluminescent Detection Kit:

• Sensitivity equal to or greater than ³²P
• Much faster than DIG detection systems
• Amenable to nearly any DNA or RNA hybridization method
• Optimized hybridization and blocking buffers and protocol steps yield consistent results from experiment to experiment
• High level of light output and consistent low background allows for very short and multiple exposure times using cooled CCD camera systems or film
• Ready-to-use buffers and short process time makes switching from radioactive detection to chemiluminescent detection an easy transition, even for the novice user
• Specific plaques can be picked out of areas that are very dense with signal due to extremely low background

The North2South Hybridization and Detection Kit combines a novel enhanced luminol substrate for horseradish peroxidase (HRP) with optimized pre-made hybridization and blocking buffers to ensure consistent results and high sensitivity. Any nucleic acid blot can be hybridized with the appropriate biotinylated (biotin-labeled) oligonucleotide probe. After a brief membrane blocking step, the biotin label is detected with streptavidin- HRP and a 5-minute incubation with the chemiluminescent substrate. Results are easily recorded by brief exposure to X-ray film (1-15 minutes) or CCD camera.

Related Products
North2South™ Complete Biotin Random Prime Labeling and Detection Kit
North2South™ Chemiluminescent Substrate for HRP
North2South™ Hybridization Buffer
North2South™ Hybridization Stringency Wash Buffer (2X)

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