- 商品介绍
- 规格参数
- 包装参数
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规格 |
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| Assay Entry: | Biochemical binding/kinetic |
| Kinase Group: | Serine/Threonine Kinase |
| Target Entry: | AMPK (A1/B1/G3) |
| Species: | Human |
| Validated Application: | Kinase Assay |
| Molecular Weight: | 63.6 /31.2/ 55.1 kDa |
| Gene Alias: | Subunit A1: PRKAA1, MGC33776, MGC57364 Subunit B1: PRKAB1, AMPK, HAMPKb, MGC17785 Subunit G3: PRKAG3 |
| Protein Length: | aa 10-end |
| Protein Tag: | His Tag |
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储存 |
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Storage Buffer: 50 mM sodium phosphate pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, 25% glycerol Store at -68 to -85°C. Aliquot at first use to avoid multiple freeze-thaws. |
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描述 |
AMPK (A1/B1/G3) is a member of the AMPK family which are heterotrimeric proteins consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPKs are an important energy-sensing enzyme group in the cells that monitor energy status particularly in response to stress. AMPKs regulate fatty acid and cholesterol synthesis by regulating the key rate-limiting enzymes acetyl-CoA carboxylase and hydroxy beta-methylglutaryl-CoA reductase. The gamma subunit is dominantly expressed in skeletal muscle where it may play a key role in the regulation of energy metabolism. Recombinant full-length human AMPK (combination of A1/B1/G3 subunits) was expressed by baculovirus in Sf9 insect cells using a C-terminal His tags.
• Validated in LanthaScreen® Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use
Related Product:
LanthaScreen® Eu Kinase Binding Assay
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