规格
Accession Number:	NP_063937.2
Assay Entry:	Biochemical binding/kinetic, Biochemical phospho-specific activity (no antibody)
Kinase Group:	Ser⁄Thr Kinases: CMGC Group
Product Line:	LanthaScreen®
Readout:	Kinetic
Shipping Condition:	Dry Ice
System Compatibility:	LanthaScreen®
Tag Position:	N-Terminal
Target Entry:	GSK3A
Expression System:	Baculovirus
Form:	Frozen
Protein Family:	Kinases & Inhibitors
Protein Form:	Full Length
Protein Subtype:	Serine⁄Threonine Kinases
Research Category:	Signal Transduction
Species:	Human
Validated Application:	Kinase Assay
Gene ID (Entrez):	2931
Molecular Weight:	78.4 kDa
Purification Method:	Purified
Gene Alias:	GSK-3A
KinaseFamily:	GSK Kinase Family
Protein Tag:	GST Tag

储存
Storage Buffer: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.5 mM EDTA, 0.02% NP-40, 2 mM DTT and 50% glycerol. Store at -80°C. At first use, aliquot and store at -80°C to avoid multiple freeze-thaws.

描述

This recombinant fusion protein consists of full-length glycogen synthase kinase 3 alpha (GSK3A) and a glutathione S-transferase (GST) tag. It is validated in the LanthaScreen® Eu Kinase Binding Assay. No special measures were taken to activate this kinase.
• Compatible and validated with the LanthaScreen® Eu Kinase Binding Assay
• Expressed in insect cells
• GST tagged for use with Eu-labeled GST antibody

Easily Screen for Kinase Binding
The LanthaScreen® Eu Kinase Binding Assay is a TR-FRET assay that lets you measure inhibitor binding to non-activated kinases, perform simple off-rate measurements and makes simple the study of type III (allosteric) inhibitors.

The LanthatScreen® Eu Kinase Binding Assay is easy to optimize and run. The assay uses a simple mix-and-read format with a single one hour incubation and no development steps. Simultaneous binding of an Alexa Fluor® conjugate or tracer” to a tagged kinase is detected by addition of a europium-labeled anti-tag antibody, in this case anti-GST. Simultaneous binding of the tracer and antibody to the kinase results in a high degree of fluorescence resonance energy transfer (FRET), while displacement of the tracer with a kinase inhibitor results in a loss of FRET (Fig 1).

GSK3A for the LanthaScreen Kinase Binding Assay
Glycogen synthase kinase 3 alpha (GSK3A) is a multifunctional protein serine/threonine kinase implicated in the control of several regulatory proteins and transcription factors. (GSK3A Alias: DKFZp686D0638)

This insect cell-expressed recombinant fusion protein of full-length GSK3A and GST tag has been validated in the LanthaScreen® Eu Kinase Binding Assay with these reagents:
• Tracer 236 (Cat No. PV5592)
• Eu-labeled GST Antibody (Cat No. PV5594)

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Link
• Learn more about the LanthaScreen® Eu Binding Assay.

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