规格
Accession Number:	NM_002566.4
Assay Entry:	Cell-based beta lactamase reporter gene
Cell Line:	CHO-K1
Cell State:	Dividing Cells
Druggable Target:	GPCRs
Gene Symbol:	P2RY11
Population Status:	Single Cell Clone Origin
Product Line:	GeneBLAzer®
Reporter Gene:	BLA (Beta-Lactamase)
System Type:	GeneBLAzer™
Target Entry:	P2RY11
Technique:	FRET
Detection Method:	Fluorescent

储存

Contents:
K1729 GeneBLAzer® P2RY11-NFAT-bla CHO-K1 cells
(LiveBLAzer™-FRET B⁄G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
• GeneBLAzer® P2RY11-NFAT-bla CHO-K1 cells
• GeneBLAzer® P2RY11-NFAT-bla CHO-K1 Assay Protocol
• Certificate of Analysis

Storage:
The GeneBLAzer® P2RY11-NFAT-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.

描述

GeneBLAzer® P2RY11-NFAT-bla CHO-K1 cells contain the human Purinergic receptor P2, G protein-coupled, 11 (P2RY11) stably integrated into the CellSensor® NFAT-bla CHO-K1 cell line. CellSensor® NFAT-bla CHO-K1 cells (Cat. no. K1534) contain a beta-lactamase reporter gene under control of the Nuclear Factor of Activated T-cells (NFAT) response element.

The GeneBLAzer® P2RY11-NFAT-bla CHO-K1 cells are functionally validated for Z' and EC₅₀ concentrations of ATP. In addition, GeneBLAzer® P2RY11-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

P2RY11 expression is broadly distributed and has been found in the brain, pituitary, lymphocytes, spleen, intestines, macrophages, lung, stomach, adipose tissue, pancreas, kidney, prostate, heart, placenta, liver, and skeletal muscle. Differentiation of human promyelocytic HL60 cells and maturation of monocyte-derived dendritic cells have been shown to be mediated by ATP activation of P2RY11.

For Academic pricing please login or contact us at discoverysciences@invitrogen.com.

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