规格
Cloning Method:	TOPO®-TA
Key Functions:	Reporter Assays
Product Line:	TOPO®
Constitutive or Inducible System:	Constitutive
Delivery Type:	Transfection
Promoter:	CMV
Reporter Gene:	GFP (Cycle 3)
Selection Agent (Eukaryotic):	Geneticin® (G-418)
Vector:	TOPO-TA Vectors
Protein Tag:	GFP (Cycle 3)

储存

GFP Fusion TOPO® Expression Kits contain two boxes. The GFP Fusion TOPO® TA Cloning box contains linearized and topoisomerase I-activated cloning vector, dNTPs, salt solution control PCR template and primers, forward and reverse primers for sequencing and PCR screening, and a GFP expression control plasmid. Store at -20°C. The One Shot ¤ box contains transformation reagents including single-use, 50-µl aliquots of One Shot® TOP10 Chemically Competent E. coli, S.O.C. medium, and supercoiled control plasmid. Store the One Shot® box at -80°C. Kits are guaranteed stable for 6 months when properly stored.

描述

The GFP Fusion TOPO® TA Expression Kits are designed to allow fusion of a protein of interest to the Cycle 3 GFP protein. These kits provide topoisomerase-I activated pcDNA3.1/NT-GFP-TOPO® or pcDNA3.1/CT-GFP-TOPO® vectors (Figure 1). Taq-amplified DNA fragments are ligated into these vectors in a simple 5-minute reaction right on your bench top. The vectors are designed for high-level expression of transient or stable GFP fusion proteins in a wide range of mammalian cells (Figure 2). Expression is easily detected in living cells using fluorescence. In addition, recombinant proteins expressed from these vectors can be detected on western blots using GFP Antiserum.

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