规格
Final Product Type:	cDNA (Labeled)
Includes Label or Dye:	Yes
Labeling Method:	Direct Labeling
Labeling Target:	cDNA
Label or Dye:	Alexa Fluor™ 555, Alexa Fluor™ 647
Product Line:	Alexa Fluor™, SuperScript™
Sample Type:	RNA
Detection Method:	Fluorescent

储存

All SuperScript® Direct cDNA Labeling Systems/Modules include SuperScript® III Reverse Transcriptase, anchored Oligo(dT), random primers, control RNA, 5X first-strand buffer, 0.1 M DTT, 10 mM dNTP mix, low-elution purification columns, loading buffer, wash buffer, amber collection tubes, and DEPC-treated water. The SuperScript® Plus Direct cDNA Labeling System/Module additionally include Alexa Fluor® 555- and 647-aha-dUTP. The 'System' also includes purification components. See the 'Module' (L101604) if purification components are not needed.

Store purification columns, loading buffer, and wash buffer at room temperature. Store all other components at -20°C. Guaranteed stable for 6 months when properly stored.

描述

The SuperScript® Plus Direct cDNA Labeling System is a microarray labeling kit that combines the superior performance of SuperScript® III Reverse Transcriptase (RT) with the simplicity of direct labeling. In place of an enzyme spike-in, the streamlined protocol incorporates balanced sets of novel, array-optimized, nucleotides conjugated to Alexa Fluor® 555 and 647 dyes. This results in more reproducible array data with higher correlation coefficients than using standalone reagents. The SuperScript® Plus Direct cDNA Labeling System provides:

• Optimized, all-inclusive direct labeling with balanced aha-Alexa Fluor® nucleotides, enabling higher numbers of array positives
• Improved correlation coefficients for more accurate data (Figure 1)
• Low elution purification columns included for ease of use

In the SuperScript® Plus cDNA Labeling method, anchored oligo(dT) anneals to the mRNA template (Figure 2). SuperScript® III RT extends from the priming site, incorporating dUTP labeled with Alexa Fluor® dye directly to produce labeled cDNA. Template RNA is degraded by base hydrolysis. Free nucleotides and other contaminants are removed using low-elution volume purification columns before hybridization to the microarray.

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