规格
Antibiotic Resistance Bacterial:	Ampicillin (AmpR)
Cleavage:	EK (Enterokinase) Recognition Site
Cloning Method:	Restriction Enzyme ⁄ MCS
Constitutive or Inducible System:	Inducible
Inducing Agent:	IPTG
Promoter:	Trc and lacO
Selection Agent (Eukaryotic):	None
Vector:	pTrc
Protein Tag:	Thioredoxin

储存
All vectors are supercoiled and are provided lyophilized. A TOP10 E. coli stab and positive expression control are provided. Store vectors at -20°C. Store stab at room temperature. The vector is guaranteed stable for 6 months when properly stored.

描述

Our pTrcHis A, B, & C vectors are designed to offer enhanced translation initiation and high-level expression in E. coli. These vectors feature:

• High-level regulated transcription from the trc promoter
• Enhanced translation efficiency of eukaryotic genes in E. coli
• The lacO operator and lacIq repressor gene for transcriptional regulation in any E. coli strain

This particular vector offers:

• C-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with an anti-His(C-term) antibody
• C-terminal c-myc epitope for easy detection of fusion proteins with an anti-myc antibody

For N-terminal polyhistidine tag and Xpress™ epitope, please see our pTrcHis A, B, & C Vector.

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