规格
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Cell Type:
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Hematopoietic Cells |
Target Species:
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Mouse |
The MagniSort™ Mouse Hematopoietic Lineage Depletion Kit is designed for the magnetic depletion of mouse hematopoietic cells with committed lineages from mouse bone marrow. It utilizes a cocktail of antibody specific for hematopoietic lineages and streptavidin-coated magnetic beads. Lineage committed cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the lineage negative cells can be separated from lineage committed cells by decanting.
The MagniSort™ Mouse Hematopoietic Lineage Depletion Antibody Cocktail contains the following antibodies:
Anti-Mouse CD2 Biotin
Anti-Mouse CD3 Biotin
Anti-Mouse CD5 Biotin
Anti-Mouse CD11b Biotin
Anti-Mouse CD19 Biotin
Anti-Mouse CD45R Biotin
Anti-Mouse Ly-6G (Gr-1) Biotin
Anti-Mouse TER-119 Biotin
After depletion, the enrichment of multi-potent progenitor cells can be verified by staining with Anti-Mouse CD34 (clone RAM34), and the enrichment of CD34- hematopoietic stem cells can be verified by additional staining with Anti-Mouse CD150 (clone mShad150) and Anti-Mouse CD48 (clone HM48-1).
Reported Application
Magnetic Cell Separation