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SUPERSIGNAL MOLECULAR WEIGHT PROTEIN LADDER,84785,赛默飞世尔

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订货号 3DC8859
品牌型号 赛默飞世尔 84785
货期 询货期
最小订货量 1套
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产品介绍 Product Description
规格
Detection Method: User defined detection system
Number of Markers: 8
Product Line: SuperSignal™
Size Range: 20 to 150 kDa
System Type: SDS-PAGE
Description: SuperSignal Molecular Weight Protein Ladder
Molecular Weight: 150, 100, 80, 60, 50, 40, 30, 20 kDa
Stain Type: Unstained
储存
Contents: one vial of 250 µL

Storage buffer: 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol

Storage: Upon receipt store at -20°C for long term storage, or three months at 4°C or one month at ambient

描述

Thermo Scientific SuperSignal Molecular Weight Protein Ladder (20 to 150K) is a mix of eight unstained recombinant proteins with IgG-binding sites for chemiluminescent, fluorescent, chromogenic or other detection systems. The protein standard is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

Compare and view all other protein standards and ladders ›

The ladder’s recombinant proteins bind to the antibodies used in the western blot through the IgG binding site. The protein markers can then be visualized using appropriate substrates for enzyme-labeled antibodies or via fluorescent dye-labeled antibodies (not recommended for antibodies labeled with fluors in the 500–550 nm channel).

Two versions of the MW marker are offered. This formulation is appropriate for use with most rabbit and other non-mouse polyclonal antibodies. SuperSignal Enhanced Molecular Weight Protein ladder is formulated specifically for applications requiring mouse monoclonal antibodies and experiments where very dilute antibody concentrations are used. For example, the enhanced standard may be necessary when using a 1:50,000 (25 ng/mL) to 1:100,000 (10 ng/mL) dilution of secondary antibody.

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