规格
Delivery Type:	Transfection
Key Functions:	Reporter Assays
Product Line:	Pierce™
Promoter:	TK
Reporter Gene:	Gaussia Luciferase
Selection Agent (Eukaryotic):	Puromycin
Cloning Method:	Restriction Enzyme ⁄ MCS
Description:	pTK-Gaussia Luc Vector

储存
Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: -70 to -20°C.

描述

The Thermo Scientific pTK-Gaussia Luc vector is a derivative of the pMCS-Gaussia Luc vector containing the Gaussia luciferase gene under the control of the Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter. This constitutive expression vector can be used as a normalization control to account for experimental variation in combination with other reporters.

Features of the pCMV-Gaussia Luc vector:

• Weak (TK) constitutive promoters for co-transfection and normalization
• Naturally-secreting Gaussia luciferase gene, optimized for high expression in mammalian systems
• Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another
• Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
• Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
• High-copy pUC bacterial DNA replication origin

The Gaussia Luc Vectors contain a gene cloned from the copepod, Gaussia princeps. The gene encodes a naturally-secreted bioluminescent Gaussia luciferase (approx. 20kDa), which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates.

These vectors are subject to a limited use label license.

More Product Data
• Highly sensitive multiplex luciferase reporter assays
• Luciferase assays in hard-to-transfect Jurkat cells
• Monitoring neuronal differentiation using multiplexed luciferase reporters
• Activation of the antioxidant response pathway by pesticide chemicals

Related Products
pMCS-Gaussia Luc Vector for Luciferase Assays
pCMV-Gaussia Luc Vector for Luciferase Assays

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