GRPR CHO-K1 DA ASSAY KIT 1 PLATE，K1637，Invitrogen

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订货号： 3AY1359

品牌型号： Invitrogen K1637

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最小订货量： 1套

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商品介绍
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产品介绍 Product Description

规格
Accession Number:	NM_005314.2
Assay Entry:	Cell-based beta lactamase reporter gene
Cell Line:	CHO-K1
Cell State:	Division-Arrested Cells
Druggable Target:	GPCRs
Gene Symbol:	GRPR
Population Status:	Single Cell Clone Origin
Product Line:	GeneBLAzer®, T-REx™
Reporter Gene:	BLA (Beta-Lactamase)
System Type:	GeneBLAzer™
Target Entry:	GRPR
Detection Method:	Fluorescent

储存

K1637 GeneBLAzer® GRPR CHO-K1 DA Assay Kit
Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).
Includes:
• GeneBLAzer® GRPR CHO-K1 DA cells
• LiveBLAzer™-FRET B⁄G Loading Kit, 70µg
• Solution D
• GeneBLAzer® GRPR CHO-K1 DA Assay Protocol
• LiveBLAzer™-FRET B⁄G Loading Protocol
• Certificate of Analysis

Storage:
The GeneBLAzer® GRPR CHO-K1 DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.

描述

The GeneBLAzer® GRPR-NFAT-bla CHO-K1 cells contain the human Gastrin Releasing Peptide Receptor (GRPR) stably integrated into the GeneBLAzer® NFAT-bla CHO-K1 cell line. GeneBLAzer® NFAT-bla CHO-K1 (Cat # K1447) contains a beta-lactamase (bla) reporter gene under control of a NFAT response element stably integrated into CHO-K1 cells.

The GRPR-NFAT-bla CHO-K1 cells are functionally validated for Z'-factor and EC50 concentrations using established ligands. In addition, GRPR-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary. Division Arrested cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.

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