规格
Includes Label or Dye:	No
Labeling Method:	Indirect Labeling
Product Line:	Ambion™, MessageAmp™
Reverse Transcriptase:	ArrayScript™
Sample Type:	Poly(A+) RNA, Total RNA

储存

• 60 µl T7 Oligo(dT) Primer (-20°C)
• 22 µl ArrayScript™ Reverse Transcriptase (-20°C)
• 22 µl RNase Inhibitor (-20°C)
• 42 µl 10X First Strand Buffer (-20°C)
• 170 µl dNTP Mix (-20°C)
• 210 µl 10X Second Strand Buffer (-20°C)
• 42 µl DNA Polymerase (-20°C)
• 22 µl RNase H (-20°C)
• 84 µl T7 Enzyme Mix (-20°C)
• 84 µl 10X Reaction Buffer (-20°C)
• 95 µl 50 mM UTP Solution (-20°C)
• 64 µl 50 mM 5-(3-amino allyl)-UTP (-20°C)
• 255 µl ATP, CTP, GTP Mix (-20°C)
• 40 µl Second Round Primers (-20°C)
• 10 µl 1 mg/ml Control RNA (-20°C)
• 1.75 ml Nuclease-free Water (any temperature)
• 400 µl Coupling Buffer (-20°C)
• 440 µl DMSO (-20°C)
• 180 µl 4M Hydroxylamine (-20°C)
• 40 ml Wash buffer (4°C or room temperature)
• 7 ml cDNA Binding Buffer (room temperature)
• 20 ml aRNA Binding Buffer (room temperature)
• 20 aRNA Filter Cartridges (room temperature)
• 40 aRNA Collection Tubes (room temperature)
• 20 cDNA Filter Cartridges + Tubes (room temperature)
• 20 cDNA Elution Tubes (room temperature)
• 10 ml Nuclease-free Water (any temperature)
• 20 Labeled aRNA Filter Cartridges + Tubes (room temperature)
• 20 Labeled aRNA Elution Tubes (room temperature)

描述

The Amino Allyl II MessageAmp™ aRNA Amplification Kit includes reagents for aRNA amplification with amimo allyl NTP incorporation. The kit includes sufficient reagents for 20 reactions. Cy™ dyes are not included.

Improved first- and second-strand cDNA synthesis
Included in the kit is ArrayScript™ RT, a rationally engineered M-MLV reverse transcriptase, which produces equivalent or higher yields of full-length cDNA compared to other enzymes. The second-strand cDNA synthesis reaction has also been optimized to be compatible with the first-strand cDNA products generated with ArrayScript™ RT to allow maximal conversion of first-strand cDNA into full-length double-stranded cDNA templates.

Increased labeling efficiency of your aRNA
The Amino Allyl MessageAmp™ II aRNA Amplification Kit incorporates amino allyl NTP into the aRNA followed by the coupling of its reactive amino group to an NHS ester label (e.g., Cy™ or another dye). This strategy offers several advantages over the direct incorporation of labeled NTPs. Direct incorporation of labeled NTPs is inefficient and results in low yields and low specific activity aRNA and high costs. Unlike lableled NTPs, amino allyl–modified NTPs are incorporated almost as efficiently as unmodified NTPs and are much less expensive than the dye coupled NTPs.

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