规格
Antibiotic Resistance Bacterial:	Ampicillin (AmpR)
Cloning Method:	Seamless Cloning
Maximum Size:	13 kb total (vector plus all inserts)
Number of Fragments:	Up to 4 Fragments
Product Line:	GeneArt®
Replication Origin:	pUC Origin
Shipping Condition:	Dry Ice
Structure:	Linear
Reporter Gene:	Beta-Gal (lacZ)
Vector:	Seamless Cloning Vectors

储存
The product contains 5 vials of linear pUC19L vector at a concentration of 50ng⁄ml. Each vial contains enough plasmid for 4 cloning reactions using the GeneArt® Seamless Cloning and Assembly Kit (A13288 - must be purchased separately). Store the product at -80°C. The vector is guaranteed stable for 6 months when properly stored.

描述

The GeneArt® Linear pUC19L Vector is a ready-to-use, linear version of the popular cloning vector pUC19. The pUC19L plasmid (2.7 Kbp), is specifically designed as a cloning and DNA assembly vector for the GeneArt® Seamless Cloning and Assembly Kit (cat # A13288). The vector is manufactured by digesting supercoiled pUC19 plasmid with the restriction enzymes PstI and KpnI, and tested for cloning of up to 4 DNA fragments.

Some of the key features and elements of the pUC19L vector include:
• lacZα gene allows easy blue⁄white colony screening for selection of recombinants
• pUC origin of replication (high copy vector)
• Ampicillin resistance for selection in E. coli
• 6 convenient and validated restriction sites flanking the cloning region
• Tested for functionality by assembly with 4 DNA fragments using the GeneArt® Seamless Cloning and Assembly Kit

长度(mm)
宽度(mm)
高度(mm)
重量(kg)