规格
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Assay Entry:
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Cell-based beta lactamase reporter gene |
Cell State:
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Dividing Cells |
Druggable Target:
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Cytokines, Signaling Pathway |
Product Line:
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CellSensor™ |
System Type:
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CellSensor™ |
Target Entry:
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TLR3/TBK1/IRF3 pathway, TBK1, IRF3, TLR3 |
储存
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CellSensor® Cell Lines are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use. |
The CellSensor® ISRE-bla RA-1 Cell Line contains the beta-lactamase (bla) gene under the control of the interferon-stimulated response element (ISRE). This cell line was engineered by lentiviral transduction of an ISRE-bla construct into RA-1 cells. Flow cytometry was then used to isolate a pool of cells responsive to polyinosinic-polycytidylic acid (poly I:C) stimulation. ISRE-bla RA-1 cells have been tested for assay performance using variable assay conditions, including DMSO concentration, cell number, stimulation time, substrate loading time, as well as validated for Z'-factor and EC50 concentrations of poly I:C. Additional testing data using alternate stimuli are also available. Academic and non-profit customers, please inquire for special pricing.