规格
Assay Entry:	Biochemical europium phospho-specific activity
Detection Method:	Fluorescent
Excitation/Emission:	340⁄665
Form:	Liquid
Label or Dye:	Alexa Fluor™ 647
Product Line:	LanthaScreen®
Shipping Condition:	Dry Ice
Substrate:	Random (Glu:Tyr) polymer in a ratio of 4:1
Substrate Properties:	Peptide-Based Substrate
Target Entry:	ABL1 G250E, CSF1R, CSK, EPHA5, KIT, PDGFRA V561D, SYK, TYRO3, ZAP70, FGFR1, FGFR2, EGFR, EPHB2, FGFR4, IGF1R, LYNA, MATK, MERTK, NTRK3, RET V804L, TEK, FLT1, MUSK, SRCN1, ABL1 E255K, EPHA4, FYN, PTK2, PTK2B, RET, ABL1 T315I, EPHA3, EPHB4, FLT3, FLT4, INSR, LCK, YES1, EGFR L858R, ERBB4, KDR, MET, TNK2, ABL1, ABL2, AXL, FGFR3, FGR, HCK, KIT V654A, EPHA1, EPHA8, EPHB3, INSRR, LYNB, NTRK1, PDGFRA D842V, SRMS, EGFR L861Q, EPHA2, FER, LTK, PDGFRA, EPHA7, EPHB1, FRK, ITK, FLT3 D835Y, SRC, BTK, FES, ERBB2, NTRK2
Target Enzyme:	Tyrosine Kinases
Technique:	TR-FRET
Validated Kinases:	EGFR
Substrate Type:	Kinase Substrate
Color:	Far-Red
Quantity:	1nmol

储存
30 µM AlexaFluor 647 Poly-GT peptide substrate in 10 mM HEPES, 137 mM NaCl, 2.7 mM KCl, pH 7.5 (HEPES buffered saline). Store at -20°C and protect from light.

描述

A general AlexaFluor647-labeled peptide substrate consisting of a polymer of glutamate, and tyrosine that is used to monitor tyrosine kinase acitivty.
For use in combination with the LanthaScreen® Eu-PY20 Antibody (PV5696) to evaluate inhibitors in medium to high throughput screening applications.

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