DYE & BIOTN REMV SPN CLM,2 ML 25 COLUMNS，A44298，赛默飞世尔

The Thermo Scientific Pierce Dye and Biotin Removal columns enable fast and efficient removal of non-reacted fluorescent dyes, biotin, reducing agents, and crosslinkers from protein samples. Removing free-dye after a labeling reaction is often difficult and time-consuming but is essential for accurate determination of dye-to-protein ratios. The Pierce Dye and Biotin Removal resin is highly specialized to produce exceptional protein recoveries while effectively removing non-conjugated fluorescent dyes, biotinylation reagents, reducing agents, and crosslinkers. Using the appropriate amount of sample and buffer conditions, almost any fluorescent dye can be removed with these columns. Even dilute protein samples (25 µg/mL) can be successfully processed to remove dyes, biotin, reducing agents, and crosslinkers and to efficiently recover proteins (>7 kDa). A single 2-mL Dye and Biotin Removal Spin Column can process a 400 to 700 µL of sample. This product is not recommended for desalting or for buffer exchange

Features of Pierce Dye and Biotin Removal columns:
• Versatile—removes unreacted fluorescent dyes, biotinylation reagents, crosslinkers, and reducing agents from protein solutions
• High recovery—low-binding resin maximizes protein recovery
• Easy-to-use—no cumbersome column preparation or equilibration
• Fast—small-molecule removal and protein recovery in less than 15 mins
• Flexible—available in spin columns and filter spin plates for a range of needs

Available formats:
• Spin columns: 0.5, 2, 5, and 10 mL
• 96-well filter plates: pre-dispensed; compatible with centrifugation for manual or automated purification; enable fast, consistent well-to-well and plate-to-plate reproducibility for small-scale, high-throughput clean-ups

Applications:
• Ideal for post-reaction clean-up of samples
• To effectively remove non-conjugated dye, biotin, reducing agents, and crosslinkers with exceptional protein recovery