MED 199 EARLES 10X1L，31100035，Invitrogen

规格
Form:	Powder
Glucose:	Low Glucose
Glutamine:	L-Glutamine
HEPES Buffer:	No HEPES
Phenol Red Indicator:	Phenol Red
Product Line:	Gibco®
Shipping Condition:	Room Temperature
Sodium Pyruvate Additive:	No Sodium Pyruvate
Quantity:	10 x 1L

储存

Storage conditions: 2-8° C Shipping conditions: Ambient Shelf life: 36 months from date of manufacture

描述

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco® Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

The complete formulation is available.

Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO₂ incubator, or with Hanks' salts for use without CO₂.

Dual-Site cGMP Manufacturing
Gibco® Medium 199 is manufactured in a cGMP New York facility that is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 . For supply chain continuity, we offer an identical Gibco® Medium 199 product made in the Scotland facility (31100-019). This second cGMP manufacturing location is also registered with the FDA as a medical device manufacturer and is certified to ISO 13485. Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO₂ environment to maintain physiological pH. Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).