规格
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High-throughput Compatibility:
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High-throughput Compatible |
Isolation Technology:
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Magnetic Bead |
Prep Scale:
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< 100 µl Blood Samples (Small-Scale) |
Product Line:
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DYNAL®, Dynabeads® |
Purification Target:
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Genomic DNA |
Shipping Condition:
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Room Temperature |
Sample Type:
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Blood |
For Use With (Application):
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PCR, Southern Blotting, Sequencing, Cloning |
储存
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The kit contains: Dynabeads® MyOne™ SILANE (5 ml, 40 mg/ml, see Cat. no. 370-02D for further characteristics), binding/washing/elution buffers and a protocol optimized for isolation of genomic DNA. The kit content is sufficient for 96 isolations. Store at: 2-8°C. |
For highly predictable and consistent extraction and isolation of genomic DNA from human whole blood, following a simple separation protocol. Isolates linear DNA yields relative to the number of white blood cells (WBC) present in the sample. The 1 µm Dynabeads® have an optimized silica-like surface chemistry and a high specific surface area, providing efficient kinetics and a high capacity.
Their uniform size and surface area ensure highly reproducible results and an improved performance compared to magnetic particles from alternative suppliers. Other particles often have a random size range distribution, surface area and binding capacity. This could compromise the reproducibility of your results. Dynabeads® hold reputable Dynal high standards with respect to within- and between-lot reproducibility and automation ability.
Advantages:
• Highly predictable binding per mg of beads
• Linear range of DNA yield relative to WBC-count
• High integrity and purity of isolated DNA
• Isolates 10 µg DNA from a 350 µl blood sample using 2 mg (50 µl) Dynabeads®
• Quicker than spin-columns
• More cost-effective, yet performs to the same level as spin-columns
• Automation-friendly (slow sedimentation rate + high magnetic mobility)
Applications
• Isolation of genomic DNA
• From cells and fresh/frozen blood samples
• From blood with a wide range of anticoagulants
• Well suited for automated assays
• Separate kit available for the isolation of viral DNA/RNA (Cat. no. 370-11D)
Additional Info:
Validation and customization (bead, buffer, protocol or format) can be made available on a custom OEM basis. For further information, please contact us at: ivd@invitrogen.com