RIBOMINUS(BACTERIA) 12 REACTIONS，K155004，Invitrogen 供应商、价格、厂家、报价、规格参数

产品介绍 Product Description

规格
Final Product Type:	Transcriptome RNA
For Use With (Application):	Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting, cDNA Library Construction
Format:	Magnetic Bead
Isolation Technology:	Magnetic Bead
No. of Reactions:	12 Reactions
Quantity:	1
Sample Type:	Total RNA (Bacterial)
Yield:	1 µg
High-throughput Compatibility:	Not High-throughput Compatible (Manual)
Purification Target:	Transcriptome RNA
Purification Time:	1.25 hrs
Starting Material Amount:	Up to 10 µg total RNA
System Type:	RiboMinus
Product Line:	RiboMinus™

储存

The RiboMinus™ Isolation Kits include RiboMinus™ Magnetic Beads, RiboMinus™ human/mouse, bacteria, or yeast probe, and Hybridization Buffer. The optional RiboMinus™ Concentration Module contains spin cartridges, wash tubes, recovery tubes, Binding Buffer, Wash Buffer, and RNase-free (DEPC-treated) water. Store the isolation modules at +4°C and the concentration module at room temperature. All reagents are guaranteed stable for 6 months when properly stored.

描述

RiboMinus™ Transcriptome Isolation Kits are novel purification systems that enrich the whole spectrum of RNA transcripts by depleting large ribosomal RNA molecules in a total RNA sample. These kits use a 5µ biotin-labeled, Locked Nucleic Acid (LNA) probe specific for large rRNA to remove 95-98% of rRNA molecules from human, mouse, bacteria, or yeast total RNA (Figure 1). This removal increases the representation of RNA transcript species, including non-poly(A) tailed, pre-processed RNA, small tRNAs, siRNA, snRNA, microRNA, fragmented mRNA, and other regulatory RNAs that cannot be purified via poly(A) selection methods. The RiboMinus™ Transcriptome Isolation Kits enable:

• Comprehensive analysis of the whole transcriptome without contamination or interference from rRNA
• More complete collection of transcribed elements of the genome—no reliance on poly(A) purification methods

• Cleaner, clearer microarray analysis due to higher signal-to-background ratios (Figure 2)

Purified RNA is concentrated and suitable for numerous downstream applications, including microarray analysis (Figure 2), library construction, and qRT-PCR.

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