规格
Detection Method:	Primer-Probe Detection
For Use With (Equipment):	QuantStudio™ 12k Flex, StepOne™, Fast Mode, ViiA™ 7 System
Form:	Dried Down
Format:	96-well plate
No. of Reactions:	96 Reactions
Product Line:	TaqMan™
Reaction Speed:	Fast
Research Category:	Stem Cell Research
Species:	Human

储存
Contents: Two 96-well plates each containing the 96-assay panel and two optical covers Store at room temperature.

描述

The Applied Biosystems® TaqMan® hPSC Scorecard™ Panel 2 x 96w FAST enables verification of pluripotency and determination of lineage bias for both ES and iPS cell lines. Each of the two 96-well plates contains 94 predefined TaqMan® Gene Expression assays (including endogenous controls) dried-down in the wells. Master mix is not included with this panel. For a kit that combines the panel with master mix, see TaqMan® hPSC Scorecard™ Kit 2 x 96w FAST. This product is also available in a 384-well format.

• Assess an entire gene signature in one simple experiment
• Compare results to a reference standard with the accompanying hPSC Scorecard™ Analysis Software
• Identify lines with trilineage differentiation potential
• Evaluate inductive differentiation specified to one germ layer
• Access validated content for increased confidence in your results

Assess an Entire Gene Signature in One Experiment
The TaqMan® hPSC Scorecard™ Panel helps save time by offering pre-plated assays in a stable and convenient dried-down format. Simply add TaqMan® Master Mix to your cDNA of interest and transfer to the 96-well plate with a multichannel pipette.

Compare Results to a Reference Standard
The TaqMan® hPSC Scorecard™ Panel includes access to proprietary cloud-based analysis software that allows you to view and export graphs and tables of results, as well as generate reports. The software is compatible with seven Applied Biosystems® qRT-PCR systems and is available at no additional charge.

Identify Lines with Trilineage Differentiation Potential
Include randomly differentiated embryoid body (EB) samples (differentiated for at least 7 days using standard EB methods) in your analysis and determine whether your lines are biased toward one or more of the three germ layers (endoderm, mesoderm, ectoderm).

Evaluate Inductive Differentiation Specified to One Germ Layer
The panel includes over twenty markers for each of the three germ layers that have been shown to respond as expected to directed differentiation in monolayer culture. Quickly evaluate time course data, culture conditions and media formulations for your germ layer of interest with this one panel.

Access Validated Content
The panel contents are based on published work (Bock et al., Cell 144, 439–452, 2011) and were validated against multiple human ES and iPS lines.

What You Get
The TaqMan® hPSC Scorecard™ Panel 2 x 96w FAST includes:

• Two 96-well plates of 94 TaqMan® Gene Expression assays
• Two optical plate covers

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