Transfer SKU T4 RNA LIGASE 2500 U (5 U/UL) EACH，AM2141，赛默飞世尔

Ambion® T4 RNA Ligase catalyzes the formation of a phosphodiester linkage between a 5'-phosphoryl-terminated nucleic acid donor and a 3'-hydroxyl-terminated nucleic acid acceptor. Substrates include RNA, DNA, and oligonucleotides. One tube containing 2,500 U (5 U/ µL) is provided along with 10X Reaction Buffer. The enzyme can be used for tagging the 5' ends of mRNA with oligonucleotides for mapping studies (RACE), 3'-end labeling RNA molecules, and circularizing RNA and DNA molecules.

Unit Definition:
One unit catalyzes the formation of 1 nmol of [5'-³²P]-rA_12-18 into a phosphatase-resistant form in 30 min at 37°C.