- 商品介绍
- 规格参数
- 包装参数
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规格 |
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| Cloning Method: | Restriction Enzyme ⁄ MCS |
| Product Line: | DES® |
| Promoter: | MT |
| Vector: | pMT |
| Protein Tag: | His Tag (6x), V5 Epitope Tag |
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储存 |
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• 20 µg each of pMT/BiP/V5-His A, B, and C vectors, lyophilized in TE Buffer (pH 8.0) • 20 µg of pMT/BiP/V5-His/GFP, lyophilized in TE Buffer (pH 8.0) Store vectors at -20°C. |
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描述 |
The DES®-Inducible/Secreted Kit provides the vector pMT/BiP/V5-His for inducible, secreted expression of recombinant proteins. This vector offers the inducible metallothionein promoter that is induced upon addition of copper sulfate or cadmium chloride. The N-terminal signal sequence from the insect BiP gene is provided to direct the recombinant fusion protein through the secretory pathway of S2 cells into the culture medium. The pMT/BiP/V5-His vector offers the following additional features:
• Small size (3.6 kb) to improve DNA yields and increase subcloning efficiency
• C-terminal V5 epitope tag for rapid detection with Invitrogens Anti-V5 Antibody
• C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin
To facilitate cloning, a set of three vectors-A, B, and C-is provided. Each vector has the multiple cloning site in a different reading frame relative to the BiP signal sequence.
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| 重量(kg) |
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¥ 56581.65
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¥ 70526.15
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¥ 67162.32
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¥ 17652.29
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¥ 39676.71
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¥ 205945.25
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¥ 14722.42
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¥ 746.00
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