OMNIMAX2 CHEMICALLY COMP CELLS 20 RXN，C854003，Invitrogen

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订货号： 2LX7206

品牌型号： Invitrogen C854003

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最小订货量： 1套

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商品介绍
规格参数
包装参数

产品介绍 Product Description

规格
Bacterial or Yeast Strain:	OmniMAX™2
Blue/White Screening:	Yes
Cloning Methylated DNA:	Yes
Cloning Unstable DNA:	Not Suitable for cloning unstable DNA
Competent Cell Type:	Chemically Competent
F'episome (to make ssDNA):	Contains F' Episome
High-throughput Compatibility:	Not High-throughput Compatible (Manual)
Plasmid:	May be used for plasmids > 20 kb
Preparing Unmethylated DNA:	Not Suitable for preparing unmethylated DNA
Product Line:	One Shot™
Propagating ccdB Vectors:	Not for ccdB vector propagation
Reduces Recombination:	Yes
Shipping Condition:	Dry Ice
T1 Phage - Resistant (tonA):	Yes
Transformation Efficiency:	> 5x10^9
Transformation Efficiency Level:	High Efficiency (> 10^9 cfu⁄µg)
Format:	One Shot
Improves Plasmid Quality:	Yes
Species:	E. coli

储存
Contains: • One Shot® OmniMAX™ 2 T1R E. coli: 21 vials, 50 µl each • pUC19 DNA (10 pg/µl): 1 vial, 50 µl • S.O.C. medium: 1 bottle, 6 ml Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.

描述

The One Shot® OmniMAX™ 2 T1^R E. coli strain is an improved chemically competent cell line, perfect for use in all cloning applications, including the Gateway® technology. OmniMAX™ 2 T1^R cells offer the highest transformation efficiency of any chemically competent cells in a One Shot® format, with >5 x 10⁹ transformants/µg pUC19. They also provide efficient transformation of highly methylated DNA, since OmniMAX™ 2 T1^R cells lack the E. coli K12 restriction systems (mcrA Δ(mrr hsdRMS-mcrBC)). In addition, the strain carries the tonA genotype, which confers resistance to T1 and T5 phage infection. This protects your samples and minimizes the possibility of downtime in your lab due to phage contamination. The highly versatile OmniMAX™ 2 T1^R strain offers the following benefits:

• Ideal for transformation of Gateway® and TOPO® reactions
• Resistance to T1 and T5 phage (tonA)
• Construction of more representative genomic libraries due to the elimination of mcrA, mcrBC, mrr, and hsdRMS
• Blue/white screening of recombinant clones (lacZΔM15)

Genotype: F´ {proAB lacI^q lacZΔM15 Tn10(Tet^R ) Δ(ccdAB)} mcrA Δ(mrr hsdRMS-mcrBC) Φ 80(lacZ)ΔM15 Δ(lacZYA-argF)U169 endA1 recA1 supE44 thi-1 gyrA96 relA1 tonA panD

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