EASYPEP MAXI MS SP KIT 8 SAMPLES，A45734，赛默飞世尔

The Thermo Scientific EasyPep Maxi MS Sample Prep Kit provides pre-formulated reagents and a robust method for the preparation of high-quality samples for proteomic MS analysis to accommodate larger sample amounts.

Features of the EasyPep Maxi MS Sample Prep Kit include:
• Complete—includes pre-formulated reagents for lysis through digestion with peptide clean-up columns
• Optimized—streamlined protocol and reagents minimize the number of steps and time needed to process samples
• Flexible—reagents and protocol have been verified using cells and tissues for larger sample amounts ranging from 0.5 to 2 mg
• Time-saving—sample processing time reduced from more than one day to less than four hours
• Compatible—final preparation is ready for direct MS analysis and other downstream applications, including TMT labeling

The EasyPep Maxi MS Sample Prep Kit enables efficient and reproducible processing of cultured mammalian cells and tissues for proteomic MS analysis. The kit contains pre-formulated buffers, MS-grade enzyme mix, peptide clean-up columns, and an optimized protocol to generate MS-compatible peptide samples in less than four hours. The kit is optimized to process protein sample sizes from 0.5 to 2 mg with a high yield of MS-ready peptides ideal for phosphopeptide and other applications that require larger starting sample amounts.

The sample preparation of peptides for MS analysis is complex, with numerous steps and varied protocols, resulting in variability in both quality and quantity of peptides. This kit has been designed to improve reproducibility while saving hands-on and processing time. MS sample preparation has been simplified and standardized through the addition of Universal Nuclease to reduce viscosity from nucleic acids (replacing sonication), a rapid 'one pot' reduction/alkylation solution for cysteine modification (carbamidomethylation, + 57.02), and a trypsin/Lys-C protease mix for more complete digestion. In addition, the kit includes Peptide Clean-up columns and buffers to prepare detergent-free peptide samples for MS analysis. The optimized reagents and protocol produce high-quality peptides that are directly compatible with TMT labeling, phosphopeptide enrichment, fractionation, or are ready for MS analysis.