规格
|
No. of Wells:
|
15 wells |
Quantity:
|
10 gels |
Thickness (Metric):
|
1 mm |
Volume (Metric) Sample:
|
15 µL |
Gel Size:
|
Mini (8 cm x 8 cm) |
For Use With (Equipment):
|
Mini Gel Tank, XCell SureLock™ Mini |
Gel Percentage:
|
10 % |
Gel Type:
|
Zymogram Gel |
Mode of Separation:
|
Molecular Weight |
Separation Range:
|
30 to 150 kDa (Tris-Glycine Buffer) |
Shipping Condition:
|
Wet Ice |
Substrate:
|
Gelatin |
Substrate Type:
|
Protease Substrate |
Well Design:
|
1D Well |
Product Line:
|
Novex™ |
储存
|
One box containing 10 gels. Store in refrigerator (2–8°C). Do not freeze. Shelf life is 4 months. |
Novex 10% Zymogram Plus (Gelatin) gels are useful for the detection and characterization of proteases that use gelatin as a substrate. The proteases are run under denaturing conditions and visualized as clear bands against a dark background using a simple renaturing, developing, and staining protocol. Zymogram gels are commonly used to detect matrix metalloproteinases. Novex 10% Zymogram Plus gels are highly sensitive, detecting as little as 5 x 10-6 units of collagenase.
Formulation: Novex Zymogram Plus gels are Tris-glycine gels with 0.1% gelatin incorporated into the gel. They are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, highly purified water, and casein or gelatin as the substrate. All have a 4% stacking gel.
Choose the right gel format for your experiments
Novex 10% Zymogram Plus gels come in a fixed concentration of 10% gelatin and three different well formats, including 10-, 12- and 15-well.
Recommended buffers: Use Novex Tris-Glycine SDS Sample Buffer and Novex Tris-Glycine SDS Running Buffer with these gels. Novex Zymogram Renaturing Buffer and Novex Zymogram Developing Buffer are also recommended.