MULTISHOT FLEXPLATE DH10B T1R EA，C6481201，Invitrogen

MultiShot FlexPlate DH10B T1^R chemically competent E. coli cells are cloning competent cells that are pre-aliquoted in a 96-well PCR plate to increase transformation productivity. The FlexPlate format can be divided down to any combination of 8-well segments for flexibility in transformation throughput.

All of our T1^R strains have the tonA (fhuA) deletion in their genotype that confers resistance to T1 and T5 phage. T1 bacteriophage spread rapidly and lyse E. coli hosts, which are commonly used for cloning and library construction. A resistant genotype offers added security to protect valuable clones and libraries. This is especially important for genome and sequencing centers where T1 phage infection would be catastrophic.

Diverse DH10B T1^R cloning capabilities
MultiShot FlexPlate DH10B T1^R competent E. coli cells are useful for many cloning applications as they are resistant to the effects of ligase and ligation buffers and provide transformation efficiencies of >1 x 10⁸ cfu/µg with control plasmid DNA. The cells are also suitable for cloning DNA that contains methylcytosine and methyladenine (i.e., genomic DNA) and ideal for cloning large plasmids and BACs.

Key features of the MultiShot FlexPlate DH10B T1^R Competent Cells include:
• mcrA, mcrBC, mrr, and hsdRMS elimination of restriction systems to allow construction of more representative genomic constructs
• tonA (fhuA) genotype to confer resistance to T1 and T5 phage
• endA1 for increased plasmid yield and quantity
• recA1 for reduced occurrence of non-specific recombination in cloned DNA
• lacZΔM15 for blue/white color screening of recombinant clones

MultiShot FlexWell format—increase transformation productivity
The FlexPlate is a 96-well PCR plate that can be used in automated liquid handling systems or divided into any combination of 8-well segments for flexibility in transformation throughput. Addition of DNA to the MultiShot FlexPlate Competent Cells can be done quickly with a multichannel pipet or a liquid handling system. After addition of DNA, the cells can be transformed efficiently by heat-shock using a water bath, heat block, or thermal cycler.

Key features of the MultiShot FlexPlate format include:
• Increase throughput—compatible with automated liquid handling platforms
• Flexible—transform in increments of 8 wells to entire 96-well plate
• Convenient—competent cells pre-aliquoted into 96-well plate, ready for transformation

Genotype: F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 lacX74 recA1 endA1 araD139 Δ(ara-leu)7697 galU galK rpsL nupG λ- tonA

Find the strain and format that you need
We offer many other E. coli strains and formats of chemically competent cells and electrocompetent cells to help meet your specific transformation needs. If you require other high-throughput transformation options, choose from our collection of MultiShot formatted comp cells or email us for custom formatting options.