规格
Accession Number:	NM_177551
Assay Entry:	Cell-based beta-arrestin recruitment
Cell Line:	U2OS
Cell State:	Dividing Cells
Cytoplasmic Tail:	Native Tail
Druggable Target:	GPCRs
Gene Symbol:	GPR109A
Population Status:	Single Cell Clone Origin
Product Line:	GeneBLAzer®, Tango™
Readout:	End Point
Reporter Gene:	BLA (Beta-Lactamase)
System Type:	Tango™
Target Entry:	GPR109A
Technique:	FRET
Detection Method:	Fluorescent

储存

Contents:
K1791 Tango™ GPR109A-bla U2OS cells
(LiveBLAzer™-FRET B⁄G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
• Tango™ GPR109A-bla U2OS cells
• Tango™ GPR109A-bla U2OS Assay Protocol
• Certificate of Analysis

Storage:
The Tango™ GPR109A-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.

描述

The Tango™ GPR109A-bla U2OS cells contain the human Nicotinic Acid Receptor (GPR109A) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ GPR109A-bla U2OS cells are functionally validated for Z' and EC₅₀ concentrations of Nicotinic Acid. In addition, Tango™ GPR109A-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

For Academic pricing please login or contact us at discoverysciences@invitrogen.com.

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