规格
Accession Number:	NM_000795
Assay Entry:	Cell-based beta lactamase reporter gene
Cell Line:	CHO-K1
Cell State:	Dividing Cells
Druggable Target:	GPCRs
Gene Symbol:	D2
Population Status:	Single Cell Clone Origin
Product Line:	GeneBLAzer®
Reporter Gene:	BLA (Beta-Lactamase)
System Type:	GeneBLAzer™
Target Entry:	DRD2, D2
Technique:	FRET
Detection Method:	Fluorescent

储存
• GeneBLAzer® D2-Gqo5-NFAT-bla CHO-K1 cells • GeneBLAzer® D2-Gqo5-NFAT-bla CHO-K1 Assay Protocol • Certificate of Analysis The GeneBLAzer® D2-Gqo5-NFAT-bla CHO-K1 cells should be stored in liquid nitrogen immediately upon receipt. LiveBLAzer™-FRET B/G Loading Kit, Solution D, and other materials are required separately; please refer to the protocol.

描述

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GeneBLAzer® D2-Gqo5-NFAT-bla CHO-K1 cells contain the human Dopamine Receptor 2 (D2) stably integrated into the GeneBLAzer® Gqo5-NFAT-bla CHO-K1 cell line. GeneBLAzer® Gqo5-NFAT-bla CHO-K1 cells (K1536) contain a beta-lactamase reporter gene under control of the Nuclear Factor of Activated T-cells (NFAT) response element and the Chimeric G protein, Gqo5.

The GeneBLAzer® D2-Gqo5-NFAT-bla CHO-K1 cells are functionally validated for Z' and EC₅₀ concentrations of apomorphine. In addition, GeneBLAzer® D2-Gqo5-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

The D2 receptor is found primarily in the brain. There are two forms of D2 which are created by alternate splicing: a short form and a long form. This assay uses the long form, although little difference in ligand binding or signaling has been seen between the two forms.

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