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APO-BRDU TUNEL ASSAY KIT EACH,A23210,Invitrogen

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订货号 1ZD3577
品牌型号 Invitrogen A23210
货期 询货期
最小订货量 1套
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产品介绍 Product Description
规格
Detection Method: Fluorescent
For Use With (Equipment): Fluorescence Microscope, Flow Cytometer
Format: Tube(s)
No. of Reactions: 60
Excitation/Emission: 495⁄519
Flow Cytometer Laser Lines: 488
Host Species: Mouse
Label or Dye: Alexa Fluor™ 488
Label Type: Alexa Fluor Dyes
Purification Method: Purified
Target Gene: BrdU
Validated Application: Flow Cytometry
Product Line: APO-BrdU™
储存
Contains positive control cells (5 mL) and negative control cells (5 mL) of a fixed human lymphoma cell line, 1 vial of TdT (45 µL), 1 vial of BrdUTP (480 µL), 1 vial of anti-BrdU Ms mAb Alexa Fluor® 488 conjugate (350 µL), 1 bottle of PI/Rnase A staining buffer (30 mL), 1 vial of reaction buffer (0.6 mL), 1 bottle of wash buffer (120 mL) and 1 bottle of rinse buffer (120 mL).

Store as directed.
描述

In collaboration with Phoenix Flow Systems, we offer the APO™-BrdU TUNEL Assay Kit which provides all the materials necessary to label and detect the DNA strand breaks of apoptotic cells. When DNA strands are cleaved or nicked by nucleases, a large number of 3´hydroxyl ends are exposed. In the APO-BrdU assay, these ends are labeled with BrdUTP and terminal deoxynucleotidyl transferase (TdT) using the TUNEL technique. Once incorporated ino the DNA, BrdU is detected using a bright and photostable green-fluorescent Alexa Fluor™ 488 dye-labeled anti-BrdU antibody.

View a selection guide for all apoptosis assays for flow cytometry.

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