规格
|
Detection Method:
|
Fluorescent |
For Use With (Equipment):
|
Fluorescence Microscope, Flow Cytometer |
Format:
|
Tube(s) |
No. of Reactions:
|
60 |
Excitation/Emission:
|
495⁄519 |
Flow Cytometer Laser Lines:
|
488 |
Host Species:
|
Mouse |
Label or Dye:
|
Alexa Fluor™ 488 |
Label Type:
|
Alexa Fluor Dyes |
Purification Method:
|
Purified |
Target Gene:
|
BrdU |
Validated Application:
|
Flow Cytometry |
Product Line:
|
APO-BrdU™ |
储存
|
Contains positive control cells (5 mL) and negative control cells (5 mL) of a fixed human lymphoma cell line, 1 vial of TdT (45 µL), 1 vial of BrdUTP (480 µL), 1 vial of anti-BrdU Ms mAb Alexa Fluor® 488 conjugate (350 µL), 1 bottle of PI/Rnase A staining buffer (30 mL), 1 vial of reaction buffer (0.6 mL), 1 bottle of wash buffer (120 mL) and 1 bottle of rinse buffer (120 mL).
Store as directed. |
In collaboration with Phoenix Flow Systems, we offer the APO™-BrdU TUNEL Assay Kit which provides all the materials necessary to label and detect the DNA strand breaks of apoptotic cells. When DNA strands are cleaved or nicked by nucleases, a large number of 3´hydroxyl ends are exposed. In the APO-BrdU assay, these ends are labeled with BrdUTP and terminal deoxynucleotidyl transferase (TdT) using the TUNEL technique. Once incorporated ino the DNA, BrdU is detected using a bright and photostable green-fluorescent Alexa Fluor™ 488 dye-labeled anti-BrdU antibody.
View a selection guide for all apoptosis assays for flow cytometry.