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SS ONE STEP RT-PCR WITH PLATINUM TAQ (100 RXNS),10928042,Invitrogen

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订货号 1YQ2322
品牌型号 Invitrogen 10928042
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最小订货量 1套
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产品介绍 Product Description
规格
Detection Method: Gel Electrophoresis
Includes: 100μL SuperScript II RT/Platinum Taq DNA Polymerase mix; 3 x 1mL 2X reaction mix; 500μL 5mM magnesium sulfate; 1mL 50mM magnesium sulfate
Quantity: 100 reactions
Sample Type: RNA
Size (Final Product): 3 kb or less
Hot Start: Built-In Hot Start
PCR Method: 1-Step RT-PCR
Polymerase: Platinum® Taq DNA Polymerase
Primer Probe Compatibility: Unlabeled Oligos
Reverse Transcriptase: SuperScript™ II
Shipping Condition: Wet Ice
Product Line: Platinum®, SuperScript™
储存
SuperScript® One-Step RT-PCR System components are listed in Table 1. Store at -20°C. Guaranteed stable for 6 months when properly stored.
描述

The SuperScript One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the convenient end-point detection and analysis of RNA molecules by RT-PCR. This system combines RT/ Platinum Taq Mix and 2X Reaction Mix. The RT/ Platinum Taq Mix contains a mixture of SuperScript II Reverse Transcriptase and Platinum Taq DNA Polymerase. The 2X Reaction Mix consists of a proprietary buffer system optimized for reverse transcription and PCR amplification, Mg2+ optimized for universal use, deoxyribonucleotide triphosphates, and stabilizers. All components for cDNA synthesis and PCR are combined in a single tube with gene-specific primers and target RNA. Reverse transcription automatically follows PCR cycling without additional steps.

Note: For superior one-step RT-PCR performance, we recommend the SuperScript IV One-Step RT-PCR System or SuperScript IV One-Step RT-PCR System with ezDNase. The SuperScript IV One-Step RT-PCR System combines the high processivity of SuperScript IV Reverse Transcriptase with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched product yields, specificity, and sensitivity in less time and for a broad target range, even with suboptimally pure RNA samples.

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