规格
|
Detection Method:
|
Gel Electrophoresis |
Includes:
|
100μL SuperScript II RT/Platinum Taq DNA Polymerase mix; 3 x 1mL 2X reaction mix; 500μL 5mM magnesium sulfate; 1mL 50mM magnesium sulfate |
Quantity:
|
100 reactions |
Sample Type:
|
RNA |
Size (Final Product):
|
3 kb or less |
Hot Start:
|
Built-In Hot Start |
PCR Method:
|
1-Step RT-PCR |
Polymerase:
|
Platinum® Taq DNA Polymerase |
Primer Probe Compatibility:
|
Unlabeled Oligos |
Reverse Transcriptase:
|
SuperScript™ II |
Shipping Condition:
|
Wet Ice |
Product Line:
|
Platinum®, SuperScript™ |
储存
|
SuperScript® One-Step RT-PCR System components are listed in Table 1. Store at -20°C. Guaranteed stable for 6 months when properly stored. |
The SuperScript One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the convenient end-point detection and analysis of RNA molecules by RT-PCR. This system combines RT/ Platinum Taq Mix and 2X Reaction Mix. The RT/ Platinum Taq Mix contains a mixture of SuperScript II Reverse Transcriptase and Platinum Taq DNA Polymerase. The 2X Reaction Mix consists of a proprietary buffer system optimized for reverse transcription and PCR amplification, Mg2+ optimized for universal use, deoxyribonucleotide triphosphates, and stabilizers. All components for cDNA synthesis and PCR are combined in a single tube with gene-specific primers and target RNA. Reverse transcription automatically follows PCR cycling without additional steps.
Note: For superior one-step RT-PCR performance, we recommend the SuperScript IV One-Step RT-PCR System or SuperScript IV One-Step RT-PCR System with ezDNase. The SuperScript IV One-Step RT-PCR System combines the high processivity of SuperScript IV Reverse Transcriptase with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched product yields, specificity, and sensitivity in less time and for a broad target range, even with suboptimally pure RNA samples.