规格
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Assay:
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Immunoassay |
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Compatible Cells:
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Mammalian Cells |
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Detection Limit:
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pg/ml to µg/ml |
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Form:
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Liquid |
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High-throughput Compatibility:
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High-throughput Compatible |
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Product Line:
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DynaLight™, NovaBright™, RapidGlow™ |
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Reaction Time:
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2 min |
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Readout:
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End Point |
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Shipping Condition:
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Wet Ice |
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Substrate Properties:
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Chemical Substrate |
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Substrate Type:
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AP (Alkaline Phosphatase) Substrate |
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Target Enzyme:
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Alkaline Phosphatase |
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Detection Method:
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Chemiluminescent |
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For Use With (Equipment):
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Luminometer (Microplate) |
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Format:
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Bottle(s) |
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Quantity:
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1 bottle(s) |
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Sample Type:
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Cell Cultures, Whole Blood, Cultured Cells, Serum |
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Sensitivity:
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1 pg⁄ml |
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Substrate:
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DynaLight™ Substrate, Alkaline Phosphatase |
储存
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1 amber bottle — Store at 2 to 8°C |
Achieve faster ELISA results with DynaLight™ Substrate with RapidGlow™ Enhancer, a ready-to-use chemiluminescent substrate formulation that has been optimized to achieve faster results in solution-based assays. The DynaLight™ Substrate with RapidGlow™ Enhancer formulation includes 1,2-dioxetane chemiluminescent substrate and a polymeric enhancer that enables ultra-sensitive immunoassay detection by alkaline phosphatase label.
Features of DynaLight™ Substrate with RapidGlow™ Enhancer:
• Faster kinetics and higher signal levels than colorimetric, fluorescent, or other chemiluminescent chemistries
• Sensitive attomole detection of purified alkaline phosphatase enzyme
• Optimized formulation for both microplate and bead-based assays
• Substrate stability of over 1 year at 4°C
• Compatibility with automated platforms with trigger solution/positions already in place
The DynaLight™ Substrate with RapidGlow™ Enhancer provides a robust chemiluminescent reaction when catalyzed by alkaline phosphatase. This reaction achieves maximum light emission within 2 minutes at 37°C and maintains a sustained signal for hours. When combined with DynaLight™ Trigger Solution, DynaLight™ Substrate with RapidGlow™ Enhancer delivers maximum signal within seconds. The environmentally-friendly DynaLight™ Trigger Solution provides maximum signal within 1 second of addition, with a longer half-life to its ground state than other chemiluminescent Flash technologies, allowing ample time to collect data reliably.
For Research Use Only. Not for human or animal therapeutic or diagnostic use.