MACS GMP Recombinant Human IL-3，170-076-110，美天旎

Interleukin 3 (IL-3) is a hematopoietic growth factor with a broad spectrum of biologic activities. These include the stimulation of the proliferation and differentiation of immature pluripotent hematopoietic stem cells and various lineage-committed progenitor cells, leading to the production of most of the major blood cell types.

MACS GMP Recombinant Human IL-3 is designed for ex vivo cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.

Biological activity

IL-3 is mainly produced by activated T cells, but is also secreted by other cell types, including mast cells, eosinophils, and keratinocytes. Beside its role as hematopoietic growth factor, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils, and macrophages.

MACS GMP Recombinant Human IL-3 can be used for a variety of applications, including the ex vivo cultivation of human plasmacytoid dendritic cells from enriched CD304 (BDCA-4)⁺ cells.^2–4

Technical specifications

• Identity/ Molecular mass: 15089 Da, determined by mass spectrometry. This corresponds to the mature form of human IL-3 (133 amino acids).
• Purity: ≥ 95 % as determined by chip electrophoresis.
• Endotoxin content: < 50 EU/mg, as determined by kinetic Limulus Amoebocyte Lysate (LAL) assay (Pharmacopoeia Europaea (Ph. Eur.)).
• Residual host cell DNA content: < 20 ng/mg, as determined by quantitative PCR specific for E. coli genomic DNA.
• Residual host cell protein content: < 1 µg/mg, as determined by E. coli HCP ELISA.

MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.

MACS GMP Products are for ex vivo cell processing only, and are not intended for human in vivo applications. For regulatory status in the USA, please contact your local representative.