规格
|
Crosslinker Type:
|
Heterobifunctional |
Labeling Method:
|
Chemical Labeling |
Product Line:
|
Pierce™ |
Reactive Moiety:
|
Maleimide, Isocyanate |
Spacer:
|
Short |
Solubility:
|
DMSO (Dimethylsulfoxide), DMF (Dimethylformamide) |
储存
|
Upon receipt store at 4°C protected from moisture. Product is shipped at ambient temperature. |
Thermo Scientific Pierce PMPI is a maleimide-and-isocyanate crosslinker for attaching compounds to sulfhydryl groups (cysteines) after conjugating the linker to hydroxyl groups on a molecule by urethane (carbamate) bonds.
Features of PMPI:
• Reactive groups: maleimide and isocyanate
• Reactive towards: sulfhydryl and hydroxyl groups
• Short (8.7A), sulfhydryl-to-hydroxyl crosslinker with an aromatic spacer arm (noncleavable)
• Maleimide group reacts with sulfhydryl groups to form stable thioether linkages
• Isocyanate reacts with non-aqueous hydroxyl groups to form carbamate linkage
• Excellent tool for conjugating hydroxyl-containing compounds such as steroids and vitamins
• Targets and reaction solutions must not contain primary amines