- 商品介绍
- 规格参数
- 包装参数
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规格 |
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| Cloning Method: | Gateway® |
| Product Line: | Gateway®, InsectSelect™ |
| Promoter: | OplE2 |
| Selection Agent (Eukaryotic): | Blasticidin |
| Vector: | pIB, pDEST |
| Protein Tag: | His Tag (6x), V5 Epitope Tag |
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储存 |
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| The vectors are provided supercoiled and lyophilized and include 6 µg of expression vector and 10 µg of an expression control. The vectors are guaranteed stable for 6 months when stored properly. |
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描述 |
The pIB/V5-His-DEST vector is designed for rapid lambda phage site-specific recombination with a Gateway® entry clone and subsequent expression in Sf9, Sf21, or High Five™ insect cells. As part of the InsectSelect™ System, pIB/V5-His-DEST features:
• The OpIE2 promoter for constitutive expression
• Blasticidin resistance gene driven by the weak GP64 promoter for selection of stable cell lines that have integrated the plasmid into a transcriptionally active site
• C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and easy purification with a nickel-chelating resin
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