Gibco® Sf9 cells are commonly used to isolate and propagate recombinant baculoviral stocks and to produce recombinant proteins. The cells originated at the USDA Insect Pathology Laboratory, from the parental IPLBSF-21 (Sf21) cell line, which was derived from the pupal ovarian tissue of the fall army worm, Spodoptera frugiperda. Gibco® Sf9 cells (frozen in Grace's Insect Medium) feature:
• Recombinant protein expression from a variety of expression systems
• Good growth in adherent or suspension culture
• Small, regular size that generates even monolayers and plaques
• Quality and performance testing

Recombinant protein expression from a variety of expression systems
High levels of protein expression in Sf9 cells can be obtained using either the BaculoDirect™ Baculovirus Expression System, the Bac-to-Bac® Baculovirus Expression System, or the InsectDirect® Expression System.

Good growth in adherent or suspension culture
Protocols for adherent or suspension growth in Gibco® Supplemented Grace's Insect Medium are available in the product manual. Cultures can easily be transferred between the two conditions for ease of workflow.

Small, regular size that generates even monolayers and plaques
Gibco® Sf9 cells generate a clean, even monolayer and plaques due to their small, round, regular size. Other cells often form more irregular monolayers and plaques.

Quality and performance testing
Each lot of Gibco® Sf9 cells is tested for cell growth and viability post-recovery from cryopreservation. In addition, the Master Seed Bank has been tested for contamination of bacteria, yeast, mycoplasma and virus and has been characterized by isozyme and karyotype analysis.

Caution: Handle as potentially biohazardous material under at least Biosafety Level 2 containment. This product contains Dimethyl Sulfoxide (DMSO), a hazardous material. Review the Material Safety Data Sheet before handling.

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