- 商品介绍
- 规格参数
- 包装参数
产品介绍
Product Description
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规格 |
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| Exonuclease Activity: | 5' - 3' |
| Fidelity (vs. Taq): | 1 X |
| GC-Rich PCR Performance: | Low |
| Hot Start: | No |
| Overhang: | 3'-A |
| Polymerase: | Taq DNA Polymerase |
| Quantity: | 3 x 500 units |
| Reaction Format: | Separate Components |
| Reaction Speed: | Standard |
| Size (Final Product): | 5 kb or less |
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储存 |
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Contains: • 300 µl Taq DNA Polymerase (5 U/µl) • 7.5 ml 10X PCR buffer (without magnesium) • 3 ml Magnesium Chloride (50 mM) Store at -20°C in a non-frost-free freezer. Stable for 6 months when stored properly. |
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描述 |
Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. The enzyme consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5´→3´ DNA polymerase activity and a 5´→3´ exonuclease activity (see figure). With Taq DNA Polymerase, you get:
Applications
Amplification of DNA from complex genomic, viral, and plasmid templates, RT-PCR, sequencing ssDNA, and cycle sequencing
Source
Native enzyme is purified from Thermus aquaticus YT1.
Unit definition
One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C.
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