规格
Assay:	Serine⁄Threonine Kinase Assay
Assay Entry:	Biochemical phospho-specific activity (no antibody)
Excitation/Emission:	405⁄465, 535
No. of Reactions:	800 Reactions
Product Line:	Z´-LYTE®
Readout:	End Point
References:	BBRC vol. 61, pp 559-563 (1974), JBC, vol 252, p 4888 (1978)
Shipping Condition:	Dry Ice
Source:	porcine pyruvate liver kinase (LRRASLG)
Substrate Properties:	Peptide-Based Substrate
Target Entry:	PRKX, PRKG2, RPS6KA5, PRKACA, PRKG1, RPS6KA4, AURKB, AURKA
Target Enzyme:	Serine⁄Threonine Kinases
Technique:	FRET
Validated Kinases:	AURKA, AURKB, PRKACA, PRKG1, PRKG2, PRKX, RPS6KA4, RPS6KA5
Label or Dye:	Methylcoumarin
Format:	384-well plate

储存
Z´-LYTE® Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.

描述

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon a porcine pyruvate liver kinase derived peptide used to study PKA substrate specificity, and containing an R-R-X-S consensus sequence, where X is any residue.

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