规格
Cloning Method:	Restriction Enzyme ⁄ MCS
Key Functions:	Regulated Expression
Product Line:	Flp-In™, T-REx™, pcDNA™
Constitutive or Inducible System:	Inducible
Delivery Type:	Transfection
Inducing Agent:	Tetracycline
Promoter:	CMV⁄TO
Selection Agent (Eukaryotic):	Hygromycin
Vector:	pcDNA
Protein Tag:	Untagged

储存
Contains 20 µg of each: pcDNA™FRT⁄TO and pcDNA™FRT⁄TO⁄CAT vectors. Store both vectors at -20°C. All reagents are guaranteed stable for 6 months when properly stored.

描述

pcDNA™FRT⁄TO vector is a 5.1 kb inducible expression vector designed for use with the Flp-In™ T-REx™ System. The control plasmid, pcDNA™5⁄FRT⁄TO⁄CAT, is also included for use as a positive control for transfection and expression in your Flp-In™ T-REx™ host cell line of choice.

The pcDNA™5⁄FRT⁄TO vector contains the following elements:

• A hybrid human cytomegalovirus (CMV)⁄TetO2 promoter for high-level, tetracycline-regulated expression of the gene of interest in a wide range of mammalian cells
• Multiple cloning site with 10 unique restriction sites to facilitate cloning the gene of interest
• FLP Recombination Target (FRT) site for Flp recombinase-mediated integration of the vector into the Flp-In™ T-REx™ host cell line
• Hygromycin resistance gene for selection of stable cell lines

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