规格
|
Cloning Method:
|
Restriction Enzyme ⁄ MCS |
Key Functions:
|
Targeted Integration |
Product Line:
|
Flp-In™, pcDNA™ |
Constitutive or Inducible System:
|
Constitutive |
Delivery Type:
|
Transfection |
Promoter:
|
CMV |
Selection Agent (Eukaryotic):
|
Hygromycin |
Vector:
|
pcDNA |
Protein Tag:
|
Untagged |
储存
|
Contains both pcDNA5⁄FRT and pcDNA5⁄FRT⁄CAT vectors. Store at -20°C |
pcDNA5⁄FRT vector is a 5.1 kb expression vector designed for use with the Flp-In™ System. This vector features: 1. CMV promoter for high level expression in mammalian cells; 2. ten unique restriction site for easy cloning; 3. FLP Recombination Target (FRT) site for Flp recombinase-mediated integration of the vector into the Flp-In™ host cell line and 4. Hygromycin resistance gene for selection of stable cell lines.