规格
|
Delivery Type:
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Transfection |
Key Functions:
|
Reporter Assays |
Product Line:
|
Pierce™ |
Promoter:
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CMV |
Reporter Gene:
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Green Renilla Luciferase |
Selection Agent (Eukaryotic):
|
Puromycin |
Cloning Method:
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Restriction Enzyme ⁄ MCS |
Description:
|
pCMV-Green Renilla Luc Vector |
储存
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Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: -70 to -20°C. |
The Thermo Scientific pCMV-Green Renilla Luc vector is a derivative of pMCS-Green Renilla Luc. It contains the luciferase gene under the control of the CMV (Cytomegalovirus) promoter, creating a constitutive expression vector that can be used as a normalization control to account for experimental variation in combination with other reporters.
Features of the pCMV-Green Renilla Luc vector:
• Strong (CMV) constitutive promoter for co-transfection and normalization
• Intracellular Green Renilla luciferase gene, optimized for high expression in mammalian systems
• Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
• Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
• High-copy pUC bacterial DNA replication origin
More Product Data
• Luciferase assays in hard-to-transfect Jurkat cells
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